The McDevitt laboratory at New York University has a long tradition of developing new medical microdevice technologies that are suitable for early disease detection. In this R61 project funded by the NIH, powerful new diagnostic tools that allow individuals to self‐test for acute phase HIV infections will be developed, optimized, and tested. The diagnostic tools here developed will allow patients previously diagnosed as HIV positive to monitor their viral load following ART interruption or check for loss of viral control due to the emergence of drug resistance. Given these diagnostics are to be used in a patient's own home (or other non‐clinical, setting), they need to be cheap, robust, and suitable for use without prior training or specialist equipment. Further, they should use a biolo